Probing the Functional Mechanism of Escherichia coli GroEL Using Circular Permutation
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چکیده
منابع مشابه
Probing the Functional Mechanism of Escherichia coli GroEL Using Circular Permutation
BACKGROUND The Escherichia coli chaperonin GroEL subunit consists of three domains linked via two hinge regions, and each domain is responsible for a specific role in the functional mechanism. Here, we have used circular permutation to study the structural and functional characteristics of the GroEL subunit. METHODOLOGY/PRINCIPAL FINDINGS Three soluble, partially active mutants with polypepti...
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ژورنال
عنوان ژورنال: PLoS ONE
سال: 2011
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0026462